HISTOLOGICAL STUDIES OF GAMMA IRRADIATION AND/ OR BACILLUS THURINGIENSIS VAR. BERLINER ON THE TESTES AND OVARIES OF THE BLACK CUT WORM, AGROTIS IPSILON (HUFN.) *
EL-NAGGAR, S.E.M.(1), EMAM, A.K.(2), ALM EL-DIN, M.M.S.(1), WESHAHY, K. (2) and IBRAHIM, R.S.H.(1)
(1) Biological
Applications Department, Nuclear Research Centre, Atomic Energy Authority,
Cairo, Egypt and
(2)
Plant Protection Department,
Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Key words: Gamma irradiation,
Agrotis ipsilon, Bioinsecticides,
Bacillus thuringiensis, Histology.
دراسات هستولوجية للتشعيع الجامى و/ أو باسيلس
ثورينجينسس
على خصى ومبايض الدودة
القارضة
سميرة السيد مصطفى النجار و عزة كمال امام و مجدى محمد شبل علم الدين
و قدرى وشاحى و رضا سيد حسن ابراهيم
خلاصـــة
تناولت الدراسة الحالية تأثير تعريض العذارى كاملة النمو للدودة القارضة Agrotis ipsilon للجرعة الإشعاعية تحت المعقمة (100جراى) وكذلك معاملة يرقات العمر الرابع بتركيزين من بكتيريا Bacillus thuringiensis var. Berliner (50 و 100 جزء فى المليون) كل على حدة أو المعاملة المشتركة لكل من الإشعاع و البكتريا على بعض التغيرات النسيجية لكل من الخصية والمبيض فى المعاملات المختلفة فى فراشات الجيل الأول.
ABSTRACT
The present study was carried out to determine the effects of the substerilizing dose of gamma radiation (100Gy) applied to the full grown pupae as well as two concentrations (50 and 100 ppm) of Bacillus thuringiensis var Berliner (Crycl A) applied to the fourth instar larvae and their combination on the histology of the testis and ovaries of the adults (male or female) black cut worm, Agrotis ipsilon (Hufn.), throughout the first filial (F1) generation.
The results indicted that the testes showed many symptoms of retardation in their growth in the form of retardation of spermatogenesis, reduction in sperm bundle numbers, degeneration and liquefaction of sperm bundles which lead to appearance of large vacuoles or retardation in sperm maturation. The data also showed that the follicular epithelial cells in the ovarioles (F1 female ovary) appeared abnormal in shape, beside their limited separation from the developing oocytes, which became shrinking, semi absorbed or completely absorbed leaving vacuoles. Otherwise, the oocyte shape became rectangular and the nurse cells were reduced in size or absent in some parts.
