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COMPARISON OF
BLOOD PROTEINS AND SOME TEAMA, F.E. Biological Applications Department, Radioisotopes Applications Division, Nuclear Research Centre, Atomic Energy Authority, Egypt. Key words: Protein fraction, T3, T4, Progesterone, Holstein dairy cows.
مقارنة بين بروتينات الدم ومستويات بعض الهرمونات فاطمة إدريس إبراهيم طعيمة خلاصـة تهدف هذه الدراسة إلى تقدير التغيرات فى بروتينات مصل الدم وأجزائها باستخدام طرق الفصل الكهربى فى الأبقار الهوليشتين خلال أشهر الحمل المختلفة مع مقارنتها بالغير حوامل وتقدير مستوى هرمونات الغدة الدرقية ثلاثى ورباعى اليود و هرمون البروجستيرون. تم أخذ عينات الدم من 40 بقرة حامل فى شهور الحمل المختلفة ومن 10 أبقار غير حوامل للمقارنة.
أوضحت النتائج أن هناك نقص معنوى فى كل من البروتينات الكلية, الألبيومين
والجلوبيولين فى الشهر الثالث والأخير من الحمل عن الشهور فى منتصف الحمل
(6.5, 3.1, 3.4 جم/100 مللى و 6.5, 3.2، 3.3جم/100 مللى) مقارنة بالغير
حوامل. وجد أيضا زيادة معنوية فى ألفا-1
جلوبيولين خلال الحمل وصلت الى 33.3% ونقص معنوى فى كل من مما سبق نستخلص أن الحمل فى الأبقار الهولشتين الحلاب يؤثر على كل من البروتينات الكلية وأجزائها المختلفة. أيضا يحدث بعض التغيرات ونقص فى الهرمونات فى شهور الحمل المختلفة متمثلا فى هرمونات الغدة الدرقية ثلاثى ورباعى اليود كما أن الزيادة فى البروجستيرون فى منتصف شهور الحمل دليل على مواصلة الحمل و الحفاظ على نمو الجنين. ABSTRACT The aim of this study is to determine the changes in serum protein and its fractions by using electrophoresis in Holstein cows during different months of pregnancy in comparison with non-pregnant cows and to determine hormonal levels including T4, T3 and progesterone hormones. The samples were taken from 40 pregnant cows during deferent months and 10 non-pregnant cows. Significant decrease in the levels of total protein, albumin and globulin were observed in the third and late month of pregnancy than in mid pregnancy where the values were 6.5, 3.1 and 3.4 g/dl for early months and 6.5, 3.2 and 3.3 g/dl for late month as compared to the non-pregnant cows. Significant increase in α-1globulin was observed during months of pregnancy by about 33.3%. The decrease in the levels of α-2, β and γ-globulins were recorded by about 10%, 45.3% and 21.6%, respectively. A marked decrease in T4 hormone (5.0 µg/dl) was observed in pregnant cows than in non-pregnant ones (7.1 µg/dl). Also, a decreasing T3 level (169 ng/dl) was recorded as compared to non-pregnant cows (221 ng/dl). High significant increase in progesterone level was recorded in the mid pregnancy until reached the maximum value (49.94 ng/ml) at the 7th month of pregnancy then declined (2.42 ng/ml) at the late month of pregnancy. In conclusion, during pregnancy of Holstein dairy cows, a decline in protein fractions and thyroid hormonal levels were recorded during different months as compared to non- pregnant cows. The opposite trend was observed in progesterone levels. The increasing progesterone level at the mid pregnancy indicated its importance in the continuation of pregnancy and maintenance of fetus against maternal rejection. |
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